|
|
|
|
| |
|
|
| Vasconcelos,R.B.; Salles,L.P.; Oliveira e Silva,I.; Gulart,L.V.M.; Souza,D.K.; Torres,F.A.G.; Bocca,A.L.; Rosa e Silva,A.A.M.. |
| Follicle cultures reproduce in vitro the functional features observed in vivo. In a search for an ideal model, we cultured bovine antral follicle wall sections (FWS) in a serum-free defined medium (DM) known to induce 17β-estradiol (E2) production, and in a nondefined medium (NDM) containing serum. Follicles were sectioned and cultured in NDM or DM for 24 or 48 h. Morphological features were determined by light microscopy. Gene expression of steroidogenic enzymes and follicle-stimulating hormone (FSH) receptor were determined by RT-PCR; progesterone (P4) and E2 concentrations in the media were measured by radioimmunoassay. DM, but not NDM, maintained an FWS morphology in vitro that was similar to fresh tissue. DM also induced an increase in the expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovary follicle wall section; FSH receptor; Gene expression; Steroidogenic enzymes; Hormone steroids; Morphological aspect. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800700 |
| |
|
|
| Lima,A.P.; Moura,M.D.; Rosa e Silva,A.A.M.. |
| Endometriosis is a progressive estrogen-dependent disease affecting women during their reproductive years. The objective of the present study was to investigate whether endometriosis is associated with stress parameters. We determined cortisol and prolactin levels in serum, peritoneal and follicular fluid from infertile women with endometriosis and fertile women without the disease. The extent of the disease was staged according to the revised American Fertility Society classification (1997). Serum and peritoneal fluid were collected from 49 women aged 19 to 39 years undergoing laparoscopy. Eighteen women had stage I-II endometriosis and 10 had stage III-IV. Controls were 21 women undergoing laparoscopy for tubal sterilization. Follicular fluid was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endometriosis; Stress; Infertility; Prolactin; Cortisol. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800015 |
| |
|
|
| Caldas-Bussiere,M.C.; Garcia,J.M.; Barbosa,J.C.; Rosa e Silva,A.A.M.. |
| Avaliou-se o papel da gonadotrofina coriônica humana (hCG) e da testosterona na produção de progesterona (P4) e 17beta -estradiol (E2) pelas células da granulosa cultivadas in vitro de folículo antral de égua. Os tratamentos usados foram: 1- controle (nenhum hormônio adicionado), 2- 1UI hCG (0,3mig/ml) e 3- 10UI hCG (3,0mig/ml). O tratamento com hCG foi realizado na presença ou não de testosterona (144ng/ml). O meio foi coletado e substituído com 0,25, 3, 6, 12, 24 e 144h de cultivo. As concentrações de P4 e E2 foram mensuradas por radioimunoensaio. Não se observou diferença entre os tratamentos 1 e 3 quanto à produção de P4 e E2; o tratamento 1 resultou em aumento da concentração de progesterona após 24h de cultura (P<0,01), mas somente em presença de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Égua hormônio luteinizante; Testosterona; Esteroidogênese; Células da granulosa. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000100009 |
| |
|
|
|
